/ mass mass spectrometry, Orbitrap is an ion trap mass analyzer consisting of an outer barrel- like electrode and a coaxial inner spindle- like electrode that traps ions in an orbital motion around the spindle. The image current from the trapped ions is detected and converted to a mass spectrum using the Fourier transform of the frequency signal. Home Q Exactive HF Hybrid Quadrupole- Orbitrap Mass Spectrometer. Q Exactive HF Hybrid Quadrupole- Orbitrap Mass Spectrometer. Resource Type: Spectrometer. Make: ThermoFisher. Features & Specs. Specifications: Mass Range: 50 to 6, 000 m/ z ( up to 8, 000 m/ z with BioPharma ing In- Solution Digestion, Peptide Fractionation, and a Q Exactive Mass Spectrometer to Analyze the Proteome of Clathrin- Coated Vesicles Georg H. Borner 1, 3 and Andrew B. Fielding 2, 3; 1 Department of Proteomics and Signal Transduction, Max Plank Institute of Biochemistry, 82152. Thermo Scientific™ Q Exactive™ Plus Orbitrap™ Liquid Chromatography- High Resolution Mass Spectrometry ( LC- HRMS) System.
Tmhe Thero Scientific™ Q Exactive™ Plus benchtop LC- HRMS combines high- performance quadrupole precursor selection with high- resolution ( up to 280, 000 FWHM) accurate- mass ( HR/ AM) Orbitrap™ detection to deliver high performance and tremendous. The Thermo Scientific™ Q Exactive™ Hybrid Quadrupole- Orbitrap™ Mass Spectrometer introduced the revolutionary concept of Quanfirmation. The Q Exactive™ Plus Hybrid Quadrupole- Orbitrap™ Mass Spectrometer takes Quanfirmation to the next level with increased performance and new options that enhance applications from confident DMPK qual. Exactive Plus & Exactive Plus EMR Benchtop Full- Scan Orbitrap Mass Spectrometers • Routine sub- ppm mass accuracy • Resolving power of up to 140, 000 ( FWHM) at m/ z 200 • Maximum scan speed of 12 Hz • Intrascan dynamic range of > 5, 000: 1 • Mass range of 50 to 6, 000 m/ z ( standard mode) or 350 to 20, 000 m/ z ( EMR mode) • Full- scan MS analysis. Q- Exactive a versatile Mass- Spectrometer t d t t th to detect growth promotors i i? Maaco o a d, 6 oe berco Blokland, 6 November RAFA. 3/ 12/ · Thermo Fisher Scientific Debuts High- Performance Benchtop Quadrupole- Orbitrap Mass Spectrometer. Thermo Scientific Q Exactive is a Compelling Alternative to Q- TOF. Traditionally, laboratories have purchased Q- TOFs for small- molecule work, such as drug metabolism studies, as well as for peptide and proteomics work, and the Q Exactive is ideal. The Orbitrap, also known as the Kingdon trap, is a type of ion trap mass analyzer employed by certain lines of mass spectrometers. Compared to its other ion trap mass analyzers, the way the orbitrap works is based upon the ion trajectories as they move through the electrode, which follow an orbital monstrates how to remove and clean the S- Lens on the Q Exactive instrument series Removing and Cleaning the S- Lens on Thermo Scientific™ Q Exactive™ Mass Spectrometers on Vimeo Why Vimeo? Q Exactive Focus ( DART) Mass Spectrometer,, Q Exactive Focus mass spectrometry with DART ( Direct Analysis in Real Time) produces mass spectra in real time, directly under ambient conditions without sample treatment, without solvent extraction or other sample preparation, even directly on surfaces as paper, glass, TLC plates. DART is a non- contact sampling method. The Thermo Q- Exactive HF ( QE) Quadrupole- Obitrap mass spectrometer is used to identify, quantify, and confirm a variety of large biomolecules, including proteins, peptides, glycans, lipids, metabolites, and drug compounds.
This instrument is equipped with: This mass spectrometer is. BIOASTER Omics’ Hub acquired a new High Resolution Mass Spectrometer Q Exactive HF. The Thermo Scientific™ Q Exactive™ HF hybrid quadrupole- Orbitrap mass spectrometer features an ultra- high- field Orbitrap analyzer which doubles its speed and resolution. This new MS instrument will complete our existing park, in order to increase our analytical capacity. Exploring ECD on a benchtop Q Exactive Orbitrap Mass Spectrometer Fort, Kyle L; Cramer, Christian N; Voinov, Valery G; Vasilev, Yury V; Lopez, Nathan I; Beckman, Joseph S; Heck, Albert J R ( ) Journal of Proteome Research, volume 17, issue 2, pp. тому · A rapid and reliable method using ultra- high- performance liquid chromatography- Q- Exactive Orbitrap mass spectrometry and multiple data- processing technologies was established to investigate the metabolites of AO in the plasma and various tissues of rats, including the heart, liver, spleen, lung, kidneys, and brain. The Q Exactive Focus offers a mass range up to m/ z and is equipped with Orbitrap mass analyzer. This Q Exactive Plus Software Manual is intended for all personnel that need to perform measurements with the Q Exactive Plus mass spectrometer, especially the key operator. This manual should be kept near the instrument to be available for quick reference. Scope of This Guide The Q Exactive Plus Software Manual includes the following chapters:. Exactive is a member of the Thermo Scientific fa mily of advanced mass spectrometer ( MS) detectors.
About This Guide This Exactive Operating Manual describes the modes of operation and principle hardware components of your Exactive instrument. In addition, this manual provi des step- by- step instruct ions for cleaning and maintaining your. What kind of mass spectrometer is Q Exactive? As shown in Figure 2, the Q Exactive hybrid quadrupole- Orbitrap mass spectrometer mainly includes an ion source, a stacked- ring ion guide ( S- lens), a quadrupole mass filter, a curved linear trap ( C- trap), a Higher Energy Collisional Dissociation ( HCD) cell, and an Orbitrap mass analyzer. When to use Thermo Scientific LTQ mass e to calibrate Thermo Scientific LTQ Series, LTQ Velos Series, the LTQ Orbitrap Series and Exactive Mass Spectrometer instruments. How to do a Mass Spec Q - Exactive session? Log in your “ Mass Spec Q- exactive” session on the CORES. Updated by 02/ Q- exactive mass spec – WVU SRF 2 2. Check log sheet to see the notes of previous users. This will inform you about anything unusual that occurred before, and solvents were used before.
Which is Thermo Scientific mass spectrometer to e to calibrate the Thermo Scientific™ LTQ Velos™ Series and LTQ Orbitrap™ Velos, Q Exactive Series and Exactive Plus Mass Spectrometer instruments. Q Exactive HF- X Hybrid Quadrupole- Orbitrap Mass Spectrometer Support Explore our “ Getting Started” and “ Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our " Guides and Tools" section to access a comprehensive portfolio of product- related support resources. Feb 02, · This text is a guide to operating the Q- exactive mass spectroscopy. A more detailed and thorough account of these procedures can be found in the Q- exactive Operating Manual. The Exactive Family • The Exactive Plus • HCD cell optional • Upgradeable to Q Exactive • Mass range to m/ z 6000 • Faster polarity switching • Rotated flatapole • New scan modes • The Q Exactive • Resolving power > 140, 000 • Parent ion selection capability • Spectral multiplexing • UHPLC and nanoflow capable. Q Exactive Plus Tune Use Q Exactive Plus Tune to operate the Q Exactive Plus mass spectrometer via the data system computer. It has the following components: • The menu bar provides drop- down menus with commands for operating the program. On its right side it shows the active user role. Q Exactive Orbitrap Mass Spectrometers ( MS) High- resolution accurate- mass LC- MS instruments for emerging needs Thermo Scientific hybrid quadrupole- Orbitrap mass spectrometers enable both targeted and non- targeted screening and characterization. This Exactive Plus Operating Manual represents the Original Operating Instructions. In addition to this manual, Thermo Fisher Scientific provides other documents for the Exactive Plus mass spectrometer that are not part of the Original Operating Instructions. Reference documentation for the Exactive Plus mass spectrometer includes the following:.
Learn more at Q Exactive Mass Spectrometer is the perfect solution if you are doing pilot studies, quality. The Q Exactive mass spectrometer, combining high- performance quadrupole precursor selection with HR/ AM Orbitrap detection, can be widely used in metabonomics, proteomics, environmental and food safety, clinical research, and forensic toxicology. This " Q Exactive" instrument features high ion currents because of an S- lens, and fast high- energy collision- induced dissociation peptide fragmentation because of parallel filling and detection modes. The image current from the detector is processed by an " enhanced Fourier Transformation" algorithm, doubling mass spectrometric resolution. Q Exactive™ hybrid quadrupole- Orbitrap mass spectrometer ( MS) is the most suitable Orbitrap platform for this application1, 2. With its unique spectrum, multiplexing capabilities and faster scan speed, the Q Exactive MS provides enough scan points for precise quantification even for narrow ultra- high- performance liquid chromatography ( UHPLC. Barcelona Food Analysis Conference - Dr Martin Hornshaw – Regional Marketing Manager from Thermo Scientific introduces Dr Olaf Scheibner, Q Exactive Pro. LTQ Orbitrap ™ Velos, Q Exactive Series and Exactive Plus Mass Spectrometer instruments. The Thermo Scientific ™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for positive mode calibration of Thermo Scientific LTQ Velos Series Mass Spectrometer instruments. PRM analyses of histones on a Q- Exactive Instrument PRM analyses were performed using a Q- Exactive hybrid quadrupole- orbitrap mass spectrometer ( Thermo Fisher Scientific). 9 μm, 25 cm length, 75 μm inner diameter).